Demo Images

Real results, from input image to molecular readout.

Scroll through two examples. Each starts from standard microscopy and ends in segmentation, virtual staining, toxicity and stress maps, signaling-pathway activity and gene expression.

Representative figures.

2D monolayer

From one brightfield image to a full molecular readout.

A single HEK293T field, imaged label-free. Nervoid segments every cell and estimates viability, toxicity, signaling-pathway activity and gene expression, in about 19 seconds.

Model CPG-XLI · HEK293T, brightfield

1,037
Cells analyzed
73.4%
Viability

go

3.7%
Toxic cells

moderate

19.3s
Processing
Step 1

Every cell, segmented

From a single brightfield image, Nervoid finds and outlines all 1,037 cells in the field, no stain required.

Brightfield HEK293T image with per-cell segmentation outlines

Representative figures.

Step 2

Virtual fluorescence stain

A predicted fluorescence stain, generated from the label-free image. No dye, no extra imaging step.

Virtual fluorescence stain predicted from a label-free image

Representative figures.

Step 3

Per-cell toxicity

Each cell is scored from healthy to dying. Here, 73.4% viable with a moderate population toxicity score.

Per-cell toxicity heatmap over the HEK293T field

Representative figures.

Step 4

Differential gene expression

Genes shifting up and down against the population baseline, read straight from the image.

Differential Gene Expression-6-4-202460123456Log2 Fold Change-Log10 P-valueCLDN5MED15GNAZTHAP7CDC45PRODHNot SigDownUp

Representative figures.

Signaling-pathway activity

Activity relative to the population mean.

  • Glycolysis98
  • Apoptosis46
  • EMT27
  • B cell activation25
  • Heat shock response18
  • Amino acid metabolism17
3D organoid

Cell-type identity and stress across a whole organoid.

A retinal organoid, 8-channel. Nervoid traces and types 3,014 cells into ten retinal identities and maps toxicity and pathway activity across the structure.

Model Retinal_DINOv2_v1.0 · Retinal organoid, 8-channel

3,014
Cells analyzed
10
Cell types

retinal identities

Bipolar
Dominant type

33.4%

Early diff.
Stage

progenitor to photoreceptor

Step 1

Every cell, traced

Nervoid finds and outlines every cell across the organoid section, with no stain.

Retinal organoid section with every cell outlined

Representative figures.

Step 2

Typed cell by cell

Each cell is classified into one of ten retinal identities, from progenitors and bipolar cells to early photoreceptors, rods and cones.

Retinal organoid colored by predicted cell type

Representative figures.

Step 3

Toxicity, spatially resolved

A toxicity readout across the full structure, so liabilities show up where they happen, not just as a single number.

Toxicity heatmap across the retinal organoid

Representative figures.

Step 4

Differential gene expression

Expression changes that separate differentiating cells from the baseline population.

Differential Gene Expression-6-4-202460123456Log2 Fold Change-Log10 P-valueRHORCVRNNRLCRXVSX2ATP1A1PAX6Not SigDownUp

Representative figures.

Signaling pathways

Activity score with direction of change.

  • Stem cell maintenance▲61
  • Phototransduction▲56
  • Synaptic transmission▼49
  • Neuronal development▼49
  • Cytoskeleton▼47
  • Ion transport▼45

See these readouts on your own images.

Send us an assay and we will run it through the same pipeline and walk through the results together.

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